You are encouraged to attend the defence. The details of the defence and attendance information is included below: 

Date: March 7, 2025
Time: 10:00 AM to 12:00 PM (PT)

Defence mode: Hybrid
In-Person Attendance: Senate Chambers, UNBC Prince George Campus  
Virtual Attendance: via Zoom (additional Zoom details at bottom of message) 

LINK TO JOIN: Please contact the Office of Graduate Administration for information regarding remote attendance for online defences. 

To ensure the defence proceeds with no interruptions, please mute your audio and video on entry and do not inadvertently share your screen. The meeting will be locked to entry 5 minutes after it begins: please ensure you are on time.  

Thesis entitled: USE OF CROP RESIDUES AS SUBSTRATES FOR THE CULTIVATION OF KING STROPHARIA (STROPHARIA RUGOSOANNULATA) MUSHROOMS

Abstract: King stropharia (Stropharia rugosoannulata) is a white rot fungus that produces nutritious edible mushrooms. The species is prized among backyard mushroom cultivators due to its ability to grow on various lignocellulosic substrates in a range of environmental conditions and compete with contaminant microorganisms. These characteristics make king stropharia a great potential tool for enhancing crop residue decomposition in northern environments while producing a valuable crop of mushrooms. However, the species is understudied and underutilized since its production parameters have never been optimized.

This study sought to determine 1) which readily available substrate (of alder chips, barley straw and hemp straw) produces the best yield of king stropharia mushrooms, 2) whether substrate impacts the nutritional content of king stropharia mushrooms, 3) how king stropharia chemically alters substrates, 4) how king stropharia alters substrate microbial communities, and 5) which spent substrate makes the best soil amendment for crop production. 

A cultivation trial was conducted at a farm in Prince George, British Columbia, Canada, from June to October 2022. Eight 1 m by 1 m wooden frames of each substrate were prepared. Five frames each of alder chips and barley straw were inoculated with king stropharia spawn and the three remaining frames served as uninoculated controls. Six frames of hemp straw were inoculated, leaving two uninoculated controls. 

Substrate samples were collected prior to inoculation and again after the cultivation period. The date, mass and count of mushrooms produced from each frame was recorded. Mushrooms samples were also collected for analysis. Mushroom and substrate samples were analysed for content of carbon, nitrogen and a suite of other elements. Mushrooms were analysed for protein and lipid content. Substrate sample lignocellulosic biomass fractions (lignin, cellulose and hemicellulose) were quantified, and substrate pH and electrical conductivity were tested. Substrate fungal and bacterial DNA were extracted, amplified, sequenced and analyzed.  

Hemp straw was the fastest and highest yielding substrate in the cultivation trial. Hemp straw is believed to have been the best performing substrate, likely due to its water-holding capacity, nutrient profile, lignin content and surface area to volume ratio compared to other substrates. There also appeared to be a distinctive bacterial consortium associated with the successful cultivation of king stropharia in barley straw and hemp straw, with high relative abundance of the genera Bacillus and Paenibacillus in these samples. Further cultivation experiments using fresh spawn are necessary to properly assess king stropharia’s yield and effect on substrates since the results of this study were impacted by spawn contamination.

All spent mushroom substrate types had beneficial properties for agricultural soil amendment, even though the substrates were not completely spent at the end of the cultivation trial. King stropharia presents a potential win-win scenario wherein farmers can produce nutritious mushrooms with their crop residues while enhancing crop residue decomposition and nutrient cycling with minimal technology and labour. Although further research is required to fully realize the potential of this species, this study demonstrates how king stropharia can contribute to sustainable agricultural practices. 

Defence Committee:  
Chair: Dr. Neil Hanlon, University of Northern British Columbia  
Supervisor: Dr. Lisa Wood, University of Northern British Columbia  
Committee Member: Dr. Michael Preston, University of Northern British Columbia  
Committee Member: Dr. Guillermo Hernandez, University of Alberta
External Examiner: Dr. Jonathan Cale, University of Northern British Columbia